DNA was extracted by the salting-out procedure.
We used two markers from the previous set (UNH130 and UNH180) and four additional microsatellite primer pairs (UNH848, UNH879, UNH898 and UNH907), developed by Carleton et al. (2002).
These markers were previously placed in linkage group LG23 with a total length of 70 cM (Lee et al., 2002) and were polymorphic in the tested family.