Materials
1. Standard Glucose Solution:
0.1 anhydrous glucose is dissolved in dissolved in distilled water and then raised the volume to 100 ml. with distilled water.
2. Dinitro salicylic acid reagent (DNS) :
a. Solution "a" is prepared by dissolving 300 g of sodium potassium tartarate in about 500 ml distilled water.
b. Solution "b" is prepared by dissolving 10 g of 3,5-dinitrosalicylic acid in 200 ml. of 2N NaOH solution.
c.The dinitrosalycilate reagent is prepared by mixing solution a & b and raising the final volum to 1 litre with distilled water.