Although cassava (Manihot esculenta Crantz) is a potential bioethanol crop, high operational
costs resulted in a negative energy balance in the earlier processes. The present study
aimed at optimizing the bioethanol production from cassava starch using new enzymes
like Spezyme Xtra and Stargen 001. The liquefying enzyme Spezyme was optimally
active at 90 C and pH 5.5 on a 10% (w/v) starch slurry at levels of 20.0 mg (280 Amylase
Activity Units) for 30 min.