Electrostatic interactions between the Ag+ and negatively charged carboxylate groups in enzymes present in the cell wall of mycelia. The silver ions are reduced by enzymes present in the cell wall leading to the formation of the silver nuclei, which subsequently grow by further reduction of Ag+ ions and accumulation on these nuclei. The TEM analysis shows the presence of some silver nanoparticles both in the cytoplasmic membrane and in the cytoplasm [24].