Seed priming
Chitosan solutions (Yuhuan Chemicals, Zhejiang,
China) of 0.25%, 0.50%, and 0.75% (w/v) concentrations
(pH 5.1) were used for seed priming. Four
replicates of 50 seeds each were used for each treatment.
Fifty seeds were placed on a 3-layer blotter
wetted with 12 ml chitosan solution in a 12-cm diameter
petri dish. The petri dishes were sealed with
parafilm. The seeds were primed at 15 °C in darkness
for 58 h (0.25%) and 64 h (0.50% and 0.75%) for
Mo17, and 48 h (0.25%) and 60 h (0.50% and 0.75%)
for HuangC, respectively. Following the priming,
seeds were dried back to their original moisture contents
at room temperature. The un-priming dry seeds
were used as control (CK)
Seed primingChitosan solutions (Yuhuan Chemicals, Zhejiang,China) of 0.25%, 0.50%, and 0.75% (w/v) concentrations(pH 5.1) were used for seed priming. Fourreplicates of 50 seeds each were used for each treatment.Fifty seeds were placed on a 3-layer blotterwetted with 12 ml chitosan solution in a 12-cm diameterpetri dish. The petri dishes were sealed withparafilm. The seeds were primed at 15 °C in darknessfor 58 h (0.25%) and 64 h (0.50% and 0.75%) forMo17, and 48 h (0.25%) and 60 h (0.50% and 0.75%)for HuangC, respectively. Following the priming,seeds were dried back to their original moisture contentsat room temperature. The un-priming dry seedswere used as control (CK)
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