However, SDME is not very stable and the droplets may be lost from the needle tip of the microsyringe during the extraction. In recent years, hollow fiber based liquid phase microextraction (HF-LPME) has been introduced by Pedersen-Bjergaard and Rasmussen to minimize organic solvent usage and overcome the problems of conventional preconcentration methods [9]. In order to enhance the extraction of hydrophilic drugs, a carrier mediated extraction based on two and three phase HF-LPME has been reported as an active transport mode of LPME, in which a carrier is added to the sample solution [10], or dissolved in the impregnation solvent, in the pores of hollow fiber [11]. Especially, due to its better clean-up of carrier mediated three phase HF-LPME, it has been applied for the extraction and determination of analytes from complex matrices, such as biological and food samples [11,12]. Following the extraction of TCs and QNs from a matrix, their identification and determination can be performed using HPLC equipped with diodearray, single ultra violet (UV), or fluorescence detection. Some methods have applied mass spectrometry detection, but they require complex and expensive instrumentation, which may not be suitable for routine monitoring of TCs [13].