Total aerobic mesophilic bacteria count was enumerated in Plate Count Agar (PCA) (Merk, Darmstadt, Germany) and incubated at 30 C for 72 h; total yeasts and molds count was enumerated in Dichloran Rose Bengal Chloramphenicol agar (DRBC) ( Merk, Darmstadt, Germany) and incubated at 25 C for 5 days