The injector temperature was 2408C, the oven temperature
programme started at 1508C and increased continuously
to 1908C at a rate of 208C/min and from 190 to 2358C at a
rate of 18C/min with a final hold of 35 min. Nitrogen was
used as carrier gas at a pressure of 70 kPa. Detector temperature
was 2608C. Peak identification was with authentic fatty
acid methyl ester standards (Supelco, Deisenhofen; Fluka;
Sigma, St. Louis, MO, USA). With this procedure all esterified
fatty acids are detected and referred to as ‘total fatty acids’.
The relative amount of each fatty acid was calculated from
the ratio of the area under the signal and total area of all identified
fatty acid methyl esters (100% method).