Culture
conditions were established using the growth of M 1f
strain (control) as criterion of selection. Figure 2 shows
the effect of culture media, pH and temperature on
bacterial growth. It is important to note that the
experimental design used (sequenced mono-factorial
fix) permitted a significant increase of number of
Colony-Forming Units (CFU). The maximun P.
fluorescens growth was achieved on a culture medium
based on polypeptone, with an initial pH of 7.5 and a
temperature of 35°C.