Column chromatography
applied to this first crude mixture using CHCl3-MeOH-H2O (65:35:10, lower phase), followed by pre-parative TLC on silica gel plates (silica gel 60 F254
Merck; plates 2020 cm, 0.25 mm layer thickness),
using this same solvent mixture for elution, gave 47 mg
of1(0.00094% w/w) (Rf 0.35).