2.6. Statistical analysis
Comparison of the serum kisspeptin, NKB and estra- diol between different physiology stages was done using Student’s t-test. Pearson correlation coefficients were cal- culated for the relationship between kisspeptin and NKB and estradiol. Final data is expressed as mean ± standard deviation (SD). Differences were considered to be signifi- cant at P < 0.05.