The sputum sample collected were inoculated in Hayflick medium along with 1% agar with antibiotics penicillin (500 units/ml), amphotericin ( 5 ug/ml) Difco Laboratories Ltd., and incubated at 37 in anaerobic condition. Further maintenance was done in tissue culture flask with Hayflick medium. Those strains with transposons were identified using isogenic mutant derivative GPM70 and they were maintained in the presence of gentamicin. Using phosphate-buffered saline(PBS) solution the attached cells are scrapped and extracted for further work.