The most important technique in micropropagation
is meristem proliferation wherein apical buds or nodal
segments harbouring an axillary bud are cultured to regenerate
multiple shoots without any intervening callus
phase (Kumar Pati et al., 2006). Meristem culture
is an efficient tool for regeneration, elimination of viruses
from infected plants and production of virus-free
seed material of different plant species (Grout and Brian,
1999; Faccioli, 2001; Rout et al., 2006). Viruses do not
have their own metabolism, and virus replication, which
occurs in a host cell, is dependent on the host cell’s protein
and nucleic acid synthesizing machineries and metabolic
pathways. Virus control is difficult because virus
replication inhibitors have shown to be toxic to the host
cell as well.
The most important technique in micropropagationis meristem proliferation wherein apical buds or nodalsegments harbouring an axillary bud are cultured to regeneratemultiple shoots without any intervening callusphase (Kumar Pati et al., 2006). Meristem cultureis an efficient tool for regeneration, elimination of virusesfrom infected plants and production of virus-freeseed material of different plant species (Grout and Brian,1999; Faccioli, 2001; Rout et al., 2006). Viruses do nothave their own metabolism, and virus replication, whichoccurs in a host cell, is dependent on the host cell’s proteinand nucleic acid synthesizing machineries and metabolicpathways. Virus control is difficult because virusreplication inhibitors have shown to be toxic to the hostcell as well.
การแปล กรุณารอสักครู่..
