10. Acetone flash vaporization system for clearing filters on glass slides (see ref. [5] for specifications or see manufacturer’s instructions for equivalent devices).
11. Micropipets or syringes, 5-μL and 100- to 500-μL.
12. Microscope, positive phase (dark) contrast, with green or blue filter, adjustable field iris, 8 to 10× eyepiece, and 40 to 45× phase objective (total magnification ca. 400×); numerical aperture = 0.65 to 0.75.