Supplementary material related to this article can be found,in the online version, at http://dx.doi.org/10.1016/j.jviromet.2013.11.005.In conclusion, this study described an mRT-PCR assay optimizedby selecting proper primer combinations and concentrations andusing a line of in vitro cultured pear plants positive to three tar-get viruses. The co-efficiency rates of 97.2–100% between uRT-PCRand mRT-PCR for the detection of three viruses in field-grown pearplants indicated that the mRT-PCR was reliable and suitable forroutinely detecting and identifying those three viruses in in vitrocultured and field-grown pear plants. It will also be useful for thedetection of the same viruses in apple trees.