Approximately 10 mg of cell mass was reacted
in a small screw-cap test tube with a solution containing
1 ml of chloroform, 0.85 ml of methanol, 0.15 ml of sulfuric acid
and 0.2 ml of internal standard (benzoic acid in methanol) for
140 min at 100 C. After reaction, 0.5 ml of distilled water was
added and the test tube was shaken vigorously for 1 min.