Identification of orthologous sequences: Complete
O. niloticus mRNA sequences were obtained from the
NCBI database for most of the analyzed genes (Table 3).
The sequences identified by BLAST had E-values ,e39.
The E-value was relatively high only for Amh. When we repeated
the search using the Amh of zebrafish (AAT77729),
we identified two tilapia sequences (ONI06JC.39_H08
and ONI05ID.39_D09) with much lower E-values (2e15
and 9e12, respectively). The two sequences were positioned
in two adjacent exons of the tilapia Amh, and one
of them was similar to the human Amh. In the RBEST
database, an Fhl2-like cDNA was found with an E-value of
e104. This cDNA showed a higher similarity to Fhl3 (E ¼
e109) in the NCBI database, which is another human
gene of the FHL family. Hence, we defined the detected
tilapia cDNA as part of the Fhl3l (Fhl3-like) gene.