The resolved proteins were transferred to a nitrocellulose membrane using a Bio-Rad rransblot apparatus at 250 mA for 1 h. The membrane was immersed in a blocking solution (5% nonfat dry milk in Tris-buffered saline) and incubated at 28°C for 1 h.
Theresolved proteins were transferred to a nitrocellulose membraneusing a Bio-Rad rransblot apparatus at 250 mA for 1h. The membrane was immersed in a blocking solution (5%nonfat dry milk in Tris-buffered saline) and incubated at 28°C for 1 h.