The flow rate was ca 2.8 L/min expanded gas as measured by a dry test meter (Model DTM 200A, American Meter Co., Philadelphia, Pa ., U.S.A.) and the solvent:feed ratio was 28:1.
The powder was then extracted with dd H2O using a Soxhlet apparatus for 72 h.
The resultant extract was lyophilized for 96 h to produce a water-soluble extract.