Samples:
Forty eight samples of ear secretions were
obtained from dogs with signs of external
otitis, by means of sterile cotton swabs.
The samples were cultivated parallelly on
blood agar containing 8-10 % defibrinated
sheep blood (Bul Bio, National Centre of
Infectious and Parasitic Diseases) under
aerobic conditions at 37оС for 24 hours and on
Sabouraud dextrose agar supplemented with chloramphenicol (0.4 g/l) and cyclohexamide
(0.5 g/l) (Difco), under the same conditions
for 2-7 days. After the incubation, grown
colonies were identified using the routine
microbiological methods. The sensitivity of
all bacterial isolates was tested to antibacterial
drugs using the disk diffusion method. The
colonies grown on Sabouraud dextrose agar
were identified as M. pachydermatis on the
basis of colonies’ morphology and the
microscopic features of Gram and Loefflerstained
smears