Transfer contents of sac to 50 mL centrifuge tube. and centrifuge 5 min at 1500 * g.Decant supernate into 100 mL beaker and discard pellet.Mix 6.0 mL 10 % and add to dialysate.Heat on hot plate,stirring until visible floc from with clear supernate.Additional increments of acid may be needed.Transfer to 50 mL centrifuge tube, and centrifuge 5 min at 1500*g.
Discard supernate,disperse pellet thoroughly , fill tube with water, mix well and centrifuge.