DPCR of STEC suggests that this method should provide a
means to detect E. coli O157:H7 and other STEC in
environmental samples. To test this, groundwater was spiked
with E. coli O157:H7. The samples were serially diluted and
each of these dilutions was used as a template forPCR(Fig. 1).
No PCR product was detected in the undiluted sample