Approximately 100 broomrape seeds were placed
separately on each of 264 discs of 1 cm diameter glass fiber
filter paper (GFFP) moistened with 120 μL of sterile distilled
water and incubated in 10 cm sterile Petri dishes in the dark at
20 °C for 11 days to promote warm stratification, which is a
conditioning period necessary for the seeds to become sensitive
to the posterior chemical signal that triggers the germination.10