not indicated to increase the enzymatic cellulose conversion of the sugarcane straw pretreated at sever conditions. Hydrothermal pretreatment is enough to obtain reasonable overall yields of the cellulose conversion (50%). According to Silva et al. (2011), the use of hydrothermal pretreatment for sugarcane bagasse was also enough to convert 50% of cellulose into glucose-rich hydrolysate.Therefore, is possible to conclude that the pretreatment step of sugarcane straw at 190◦C during 10 min lead to higher cellulose conversions. Instead of expected, the delignification step is not profitable for sugarcane straw, do not leading to improvements in the enzymatic cellulose conversion.