5.2. Plant material and chemical treatment
Arabidopsis thaliana plants were grown for 8 days in a culture tube containing 1/2 MS liquid medium at pH 5.8 under continuous light conditions at 22 °C. Culture tubes were shaken to aerate them in a shaking incubator at 110 rpm (Double SHAKER NR-30; TAITEC Ltd., Saitama, Japan). Whole seedlings were used for subsequent analyses. All chemicals used for screening for inhibitory activity were dissolved in dimethyl sulfoxide (DMSO, 29) and added to liquid medium (final DMSO (29) concentration 0.1% v/v). Seedlings were incubated for 3 h in the chemical treatments and then frozen in liq. N2.