PCR analysis of OSB2 gene in putative transgenic
rice plants
The transgenic lines with GUS positive were
analyzed for the presence of OSB2 gene in their
genome. Two of Nipponbare and 5 of Taichung 65
transgenic lines were analyzed. PCR analysis showed
that about 1,300 bp fragments were amplified,
suggesting the presence of the introduced OSB2 gene
in all transgenic rice plants (Figure 5). Transgenic rice
plants of both varieties were obtained for further analysis.
Figure 5