2.4. Solvent extraction
A total of 50 g lemongrass leaves powder was stirred with 500 mL of water at room temperature for 8 h. The extract was filtered and supernatant was collected. The extraction apparatus was mounted on a heating mantle using a clamp with water connection going in and out of condensers. The sample was kept in the thimble and dropped into the
Soxhlet tube. The solvent was placed in the round bottom flask and the solvent evaporated and passed through the tube into Soxhlet extractor. The vapor was then condensed by flow under gravity and percolated through the beds of sample to extract the oil. At the end of extraction, the solvent was distilled off and the dried residues were weighed and dissolved in the appropriate solvent and stored in refrigerator at 4 °C until use. This process was repeated for 3 solvents chloroform, methanol, and water[15].