using a BLASTn (http://
www.ncbi.nlm.nih.gov/BLAST) search and was sequenced from
both strands. It was aligned with sequences of other microsporidians
in the database using CLUSTALW 1.7 multiple alignment software
(Thompson et al., 1994). The phylogenic tree was constructed
using CLUSTAL W 1.75 program with the microsporidian Amblyospora
canadensis as an out-group since Amblyospora is from Clade 1
of the microsporidians (Vossbrinck and Debrunner-Vossbrinck,
2005). Tree topology was evaluated by bootstrap analysis using
the neighbour joining method with the program parameter set
for 1000 replicates.