The root extract suppresses intrace

The root extract suppresses intracellular lipid accumulation in induced 3T3-L1 adipocytes during the early stage of adipogenesis

When we examined the ethanol extracts from the root, stem, and leaf parts of ivy gourd for their anti-adipogenic effects, the root extract apparently lowered the lipid levels in 3T3-L1 adipocytes. The amounts of accumulated lipid in the 3T3-L1 adipocytes following the root extract treatment, measured in terms of the absorbance of the oil red O dye extracted from stained cells, were significantly and dose-dependently decreased (Figure 1). There was a smaller number of fat droplets within the mature adipocytes in the presence of the root extract compared to the untreated cells as revealed by microscopy (Additional file 1: Figure S1). Such an inhibitory effect, did not result from cell damage since toxicity of the root extract was not observed with any of the concentrations tested (Figure 1).