2.5.10. Fat
Total fat content was quantified by Soxhlet extraction with petroleum
ether (AOAC 945.16) applying previously an acid hydrolysis
(AOAC 922.06). Results were expressed as % d.m.
2.5.11. Total carbohydrates
Carbohydrates were calculated by difference following this
formula:
100 (weight in grams [protein + fat + water + ash + fibre])
Results were expressed as % d.m.
2.5.12. Free glucose
The procedure for determining the amount of glucose was performed
by using an enzymatic kit as per the manufacturer’s
instructions. Analysis was carried out in triplicate. Results were
expressed as mg/100 g SCG.