Different concentrations (25, 50, 100 and 200 µl) of methanolic (5 gm/50 ml), petroleum extracts (5 gm/50 ml) and Baraka capsule (1.62 gm/25 ml) were diluted with methanol to make up the volume 1 ml.
Similarly Nigella sativa oil (1 mg/ml) was first diluted with methanol to get concentration of 100 µg/ml. Then 10, 20, 40 and 80 µl of it was further diluted with methanol to make up the volume 1 ml.
Vortex all above samples vigorously and 25 µl of it injected and chromatograms were obtained in the same conditions as that of standard THQ.
The peak area corresponding to the Rt of standard THQ was recorded,
and content of the same was calculated from the regression equation obtained from calibration curve.