Evaluation of recent literature shows that CTLC, OPLC, RLCC, and DCCC have been rarely used since 2000.
FC is still used often but mainly as part of a multi-step isolation procedure.
The main separation technologies used in recent years are methods of liquid chromatography such as MPLC and semi-preparative HPLC, as well as CPC, mainly as high-speed counter-current chromatography (HSCCC) or high-performance centrifugal partition chromatography (HPCPC).
Multi-step chromatographic operations have mostly been used, e.g. a combination of FC for pre-purification and semi-preparative HPLC for final purification.