2.2.1. Arabidopsis cultivation and DNA extraction
NiCl2 solution with different concentrations was sterile filtered
and added to the MS medium, and the final concentrations of
Ni2+ were 50 μM, 100 μM, 150 μM, 250 μM, and 350 μM. The
Arabidopsis seedlings were treated with NiCl2 for 14 d, and the DNA
in leaf cell was extracted for subsequent analysis.
Arabidopsis DNA was extracted with Universal Genomic DNA
Extraction Kit Ver. 3.0 (purchased from TaKaRa Company). The DNA
mass was detected with UV spectrophotometer and 0.8% agarose gel