In 1991, Prasad [9] did cytological studies of anther-derived safflower regeneration process to illustrate the cause of regeneration. In 1992, Ying [20] report the first successful agrobacterium tumefaciens-mediated transformation and expression of GUS reporter gene in the regenerated transgenic safflower shoot buds through tissue culture. Later, researches about agrobacterium tumefaciens- mediated genetic transformation and plantlet regeneration for safflower became popular [22, 11, 19, 6, 25]. Rohini [11] introduced a simplified in planta culture method to obtain transformed safflower plants easily. As an appreciated result, the process of regenerating safflower was reduced considerably.