Sets of triplicate flasks (250 mL) were prepared for all effluents
under investigation. Each flask contained 90mL of effluent and
10mL Kirk’s basal nutrient medium. All the effluents had different
colors. IMT and CRT effluentswere densely colored. In an initial trial
we noted that the fungus did not show any growth in these effluents
so we used those effluents after making 1:10 (v/v) dilutions
in distilledwater irrespective of absorbance at respective max. The
mediawere set at pH 4.5 withMNaOH/M Succinic acid, autoclaved,
allowed to cool and inoculated with 5mL homogenous inoculum
suspension of C. versicolor IBL-04 in laminar air flow. The inoculated
flasks were incubated for 10 days at 30 ◦C in orbital shaker under
still culture conditions. The flasks were gently shaken manually for
1min after every 24 h for aeration and mixing.l