3. Results and discussion
3.1. Identification of isolated strains
Nine hundred LAB strains were isolated from kimchi samples.
The isolates with useful enzyme activities such as b-Gal and a-Gal
were selected first and then their bile salts resistances were
examined. The isolates were spotted onto MRS agar plates containing
X-Gal or X-a-Gal and incubated for 24 h at 30 C. Total nine
isolates showed both b-Gal and a-Gal activities and they were
spotted on the MRS agar plates containing 0.1% glycodeoxycholic
acid sodium salt and incubated for 24 h at 30 C. Four isolates (C4,
C10, C182 and F27) grew well and they were finally selected for
further experiments. They were identified by API 50CHL kit and 16S
rRNA gene sequencing results