with CIRD that tested positive for CPIV, 14 (60.9%) had a history of vaccination against CPIV. When analysis was performed comparing the prevalence of positive PCR test results for each pathogen among dogs living in private households and dogs from an animal shelter or research kennel, significantly more dogs from private households were PCR positive for B. bronchiseptica (P < 0.001; Table 3). Discussion CPIV, CRCoV and B. bronchiseptica were the only pathogens detected in dogs with acute respiratory signs in the present study and