did not change during the pre-physiologicalstage; however, it decreased from 14.8 to 14.2 during the period between 28 and 49 DAA (p < 0.05). In B-type granules, NC increased from 14.9 to 15.8 when the granules developed from 14 DAA to 28 DAA, and it decreased to 14.8 by 49 DAA