Materials and Methods
Sample preparation
The analyzed autochthonous olive varieties (Buža, Istarska
bjelica and Rosinjola) are part of collection of the Institute of
Agriculture and Tourism in Poreč, Croatia. Olive trees were
grown in the same pedoclimatic conditions and cultivated with
the same agrotechnical treatments. 5 kg of healthy olive leaves
were collected from each tree at the same stage of ripening.
Leaves were left to dry on pruned branches in the air, and under
cover, for 2 months. The branches were then crushed and the
leaf was coarsely separated by hand.