Next, we measured the composition and the content of isoflavone in tempeh (24 h fermentation) made of the granular germ of defatted-yellow soybean after the extraction of soybean oil. As shown in Table 2, an exceedingly high isoflavone content (aglycon and glucoside) of approximately 880 mg was observed in the defatted-yellow-soybean-germ tempeh. In addition, the proportion of aglycon in total isoflavone was approximately 44%. On the other hand, the isoflavone content in tempeh made of the defatted-yellow-soybean cotyledon was approximately the same as that in tempeh made of whole soybean (data not shown). A new isoflavone-enriched tempeh was then prepared by keeping thesame proportion of aglycon described above (Table 2). The new tempeh was prepared by combining the defatted-soybean germ with the defatted-soybean cotyledon at a ratio of 20:80 (%). The total isoflavone content in the isoflavoneenriched tempeh was approximately threefold that in the traditional soybean tempeh. In addition, the proportion of aglycon in total isoflavone of the isoflavone-enriched tempeh was 44%, whereas that in the soybean tempeh was 15%; the aglycon content in the isoflavone-enriched tempeh was approximately eightfold that in the soybean tempeh. From these results, the isoflavone-enriched tempeh was found to have not only a higher total isoflavone content but also a higher aglycon content than the soybean tempeh