The medium was supplemented with different types and combinations
of auxins [Pi (Picloran):2,4-D (2,4-dichlorophenoxyacetic
acid)] and cytokinins [KIN (kinetin):BAP (N6-benzylaminopurine)]
at different concentrations: 0.1, 1 and 2 mg/L. The pH of the media
was adjusted to 5.6 prior to autoclaving. Cultures were kept in a
growth chamber at 25 ◦C under a 16/8 h light/dark photoperiod
using cool-white fluorescent light (mol m−2 s−1) in order to form
calli, which were subcultured every 4 weeks.