According to EN 14214, the FAME content in biodiesel must be at
least 96.5 wt.%; therefore, the stirred process was selected for
future investigation. The FAME yield was also calculated using
GC–MS and the yield determined from GS-MS analysis agreed well
with 1H NMR spectroscopy, confirming the authenticity of the
methodology (see Appendix Table A.2 for comparison).