ConclusionsStillage and bran are the two main by-products from wheatbased biorefineries. Experimental results showed that R. oryzaeis able to prevent the growth of undesired microorganismson the mixture of wheat bran and stillage. The fungus devel-ops a very dense mycelium which binds the solid substrateparticles together forming a very compact structure. Thispotentially causes oxygen limited conditions, impeding thegrowth of other aerobic microorganisms, below the fungalmat. Additionally, results show that R. oryzae is able to reachthe substrate faster than other fungi, reducing substrate avail-ability for the consumption of undesired microorganisms.Finally, operational costs linked to this novel technique wouldbe limited to the maintenance of the stock culture, resulting insignificant savings when compared to the addition of organicacid