Twenty five milliliters of homogenized eggs were artificially inoculated with 10 cells of SE in 100 ul PBS and mixed with 225 ml of either BPW or tetrathionate brilliant green broth (TBG; Difco). The control samples received 100 ul of sterile PBS. The
egg and enrichment broth mixtures were incubated at 37 jC and sampled at 24 and 48 h, respectively. The lateral flow assay was performed as described previously using 100 ul of the individual enrichment broth cultures from each sampling time.