In this study, the degree of clinical wrinkles was objectively measured by using a device (the Visiometer) that converts the skin surface roughness to numerical values. The facial wrinkles decreased in both the lower-dose and higher-dose groups; however, no dose-response relationship was seen in this study. This may be partially attributed to the small number of subjects in this study and a relatively short time period of aloe gel supplementation. The decrease in facial wrinkles as measured by the Visiometer reflects increased type I procollagen in both dosing groups. At the gene level, lower-dose aloe was shown to increase type I procollagen mRNA in the photo-protected buttock skin, albeit without statistical significance, whereas no change was seen in the MMP-1 mRNA levels. In the higher-dose group, while aloe gel had little effect on the type I procollagen mRNA levels, it caused a significant decrease in the MMP-1 mRNA levels. As a result, both dosing groups would have a net gain of procollagen that would correlate clinically with the improvement of wrinkles, as measured by the Visiometer. Type I procollagen immunostaining also demonstrated an upregulated protein expression in both groups.