Steam Sterilization of the solution Sterilization(or sterilisation) is a term referring to any process that eliminates(removes) or kills all forms of life, including transmissible agents(such as fungi, bacteria, viruses, spore forms, etc.) present on a surface. contained in a fluid, in medication, or in a compound such as biological culture media.For our process sterilization ensures the absence of any other microorganisms in the solution that can hamper the fermentation process. In our process we have used steam sterilization. This is almost the same process as the autoclaving. The same autoclave is used here for the sterilization. The sample solution is kept in the autoclave for 15 minutes at 121"Cand thus the solution is sterilized.