2.4. TLC Fingerprint Profile
n-Hexane fraction (100 mg) of V. negundo leaves was dissolved in 40 mL of n-hexane and the volume was made up to 50 mL in a volumetric flask. This fraction was used for the TLC (thin layer chromatography) fingerprinting profile. TLC plates consisted of 10 × 10 cm, precoated with silica gel 60 F254 TLC plates (E. Merck) (0.2 mm thickness) with aluminum sheet support. The spotting device was a CAMAG Linomat V Automatic Sample Spotter (Camag