It should be noted that the fluorescent signals can be quenched
among other signals when fluorophores are positioned within the
energy transfer distance (Forster Resonance EnergyTransfer distance is approximately with in 10 nm),to result in a decrease in fluorescent signals. Since the dimensi on of BSA is approximately 1404040 Å3, even though it is totally denatured by urea and DTT,the possibility of self-quenching could not be ruled out given
the structural configuration