Effect of Different pH
The bacterial isolate J1 were grown in
conical flask (250 ml) containing 100 ml
media and were sterilized at 121
oC for 20
min.. The medium sucrose /yeast extract
broth medium were prepared with different
pH ranging from (6, 7, 8 and 9) and the
inoculated flasks were incubated at 30
oC at
150 rpm for 48 hr. and PHB was quantified.