2.3. Construction of plasmid to integrate the four genes in the PPP
The four genes (TAL1/TKL1/RKI1/RPE1) were integrated into the
non-functional site of chromosome VII (+18,248 bp). The methods
to construct pUC18-TAL1/TKL1/RKI1/RPE1 (pUC-TTRR) plasmid
were described as follows.